The axial form of 15N enriched aminocyclohexane has been detected at 173K, permitting the first direct measurement of the conformational free energy of the NH2 group.

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Journal Tetrahedron Letters
Buchanan, G.W, & Webb, V.L. (1983). Conformational analysis of aminocyclohexane using 15N nuclear magnetic resonance at low temperature. Tetrahedron Letters, 24(42), 4519–4520. doi:10.1016/S0040-4039(00)85943-2