The interactions of collagen, Croda glue and VM gelatin which are organic additives employed in the base metals industry, with a polymeric and a derivatized silica stationary phase in size exclusion chromatography are examined by a 23 factorial experimental design. Main factor coefficients indicate the presence of ion-exclusion effects for all protein solutes on both stationary phases. The propensity toward ion exclusion is greater on the silica-based stationary phase. Hydrophobic adsorption of collagen and Croda glue was evident on the polymeric phase. Collagen and Croda glue display identical behaviour on both stationary phases as suggested by their principal components. Anomalous behaviour of VM gelatin is suggested by both main factor coefficients and principal component analysis.

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Journal Journal of Chromatography A
Weatherell, C.J., & Lai, E. P. (1994). Factorial experimental design and principal component analysis of the interaction of animal glues with polymeric and silica-based stationary phases in size exclusion chromatography. Journal of Chromatography A, 669(1-2), 31–44. doi:10.1016/0021-9673(94)80334-X