We have carried out immunofluorescence labelling of two human cell types, HeLa cells and peripheral blood lymphocytes, prepared by several different fixation/permeabilization protocols using a variety of antibodies against DNA Topoisomerase II (Topo II). We have found that the distribution of Topo IIα was overall similar during interphase and mitosis to that previously reported, regardless of antibody and of sample preparation. On the other hand, the interphase distribution of Topo IIβ was quite variable, depending both on the antibody and on the method used to prepare the sample. Our interpretation of the data is that, like Topo IIα, Topo IIβ is primarily a nucleoplasmic protein, but that unlike Topo IIα, small amounts are also associated with intranucleolar chromatin.

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Keywords HeLa, immunofluorescence, interphase, lymphocytes, mitogenic stimulation, mitosis, nucleolus, nucleoplasm, Topo IIα, Topo IIβ
Persistent URL dx.doi.org/10.1002/(SICI)1097-4644(19961101)63:2<162::AID-JCB4>3.0.CO;2-W
Journal Journal of Cellular Biochemistry
Chaly, N, & Brown, D.L. (1996). Is DNA topoisomerase IIβ a nucleolar protein?. Journal of Cellular Biochemistry, 63(2), 162–173. doi:10.1002/(SICI)1097-4644(19961101)63:2<162::AID-JCB4>3.0.CO;2-W