The applicability of capillary electrochromatography (CEC) with photodiode array detection for the analysis of carbonyl hydrazones is presented. The CEC separation of thirteen hydrazones was optimized by a systematic variation of conditions using a commercially available CE system and a 3-μm porous C18-bonded silica capillary column. The separation profile obtained under optimal isocratic conditions (60% actonitrile-4% tetrahydrofuran-5 mM Tris, pH 8) is similar to those reported for gradient HPLC, with significant improvements in efficiency (to 150 000-250 000 theoretical plates/m) and analysis time (by a factor of 4). The retention time reproducibility is better than 0.2% (RSD) from run to run and 1% from day to day. The limits of detection for individual carbonyl hydrazones range between 0.1 and 0.5 μg/ml. Applications to ambient air and automobile exhaust are shown. Copyright (C) 1999 Elsevier Science B.V.

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Keywords Air analysis, Carbonyl compounds, Dinitrophenylhydrazones, Electrochromatography, Environmental analysis, Hydrazones
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Journal Journal of Chromatography A
Dabek-Zlotorzynska, E. (Ewa), & Lai, E. P. (1999). Separation of carbonyl 2,4-dinitrophenylhydrazones by capillary electrochromatography with diode array detection. In Journal of Chromatography A (Vol. 853, pp. 487–496). doi:10.1016/S0021-9673(99)00473-2