By using brine shrimp (Artemia salina) lethality test-guided fractionation, a single bioactive compound (LC50=26 ppm) was isolated from the 95% ethanol extract of the dried aerial parts of Impatiens balsamina L. and subsequently identified as 2-methoxy-l,4-naphthoquinone (MNQ). The structure of MNQ was confirmed by UV, FT-IR, MS, and 1-and 2-D NMR spectroscopy. The antimicrobial activity of MNQ was evaluated using 12 bacterial and eight fungal strains. Five gram-positive and two gram-negative bacteria as well as all eight fungi (including multi-drug resistant strains) tested were highly sensitive to MNQ. A tea prepared according to traditional methods was found to contain sufficient MNQ to account for its antimicrobial properties. Copyright

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Keywords 2-methoxy-1,4-naphthoquinone, Antibacterial activity, Antifungal activity, Artemia salina, Brine shrimp lethality test, Impatiens balsamina L.
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Journal Phytotherapy Research
Yang, X. (Xiaolong), Summerhurst, D.K. (D. Kristen), Koval, S.F. (Susan F.), Ficker, C. (Christine), Smith, M, & Bernards, M.A. (Mark A.). (2001). Isolation of an antimicrobial compound from Impatiens balsamina L. using bioassay-guided fractionation. Phytotherapy Research, 15(8), 676–680. doi:10.1002/ptr.906