Poly(methacrylic acid-ethylene glycol dimethacrylate) was prepared using 2-aminopyridine as the imprinting molecule. This molecularly imprinted polymer (MIP) was ground and packed into a micro-column for selective solid phase extraction (SPE) of 2-aminopyridine from 20μl of sample solution. Non-specific adsorption was also confirmed for a structural analogue. Interestingly one of the isomers, 4-aminopyridine, bound most strongly to the MIP. The implication of resonance and basicity of this isomer molecule can be used to explain its strong binding with the self-assembled functional methacrylic acid (MAA) monomer. The monomer template complexion process was evaluated by Scatchard plots to determine a binding constant. The binding constant value is important for predicting the selectivity of a new MIP. After optimization of the molecular recognition process, a molecularly imprinted solid phase extraction-differential pulsed elution (MISPE-DPE) method was developed for the selective determination of 4-aminopyridine in serum with an analysis time of less than 3min using a 2-aminopyridine micro-column for surrogate binding. The concentration detection limit was 0.5μg/ml, which corresponded to an absolute detection limit of 10ng. A larger sample volume of 845μl afforded a better concentration detection limit of 52ng/ml. Copyright (C) 2000 Elsevier Science B.V.

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Keywords 2-Aminopyridine, 4-Aminopyridine, Differential pulsed elution, Molecularly imprinted polymer, Solid phase extraction
Persistent URL dx.doi.org/10.1016/S0003-2670(00)00837-0
Journal Analytica Chimica Acta
Mullett, W.M. (Wayne M.), Dirie, M.F. (Mohamed F.), Lai, E. P, Guo, H. (Hongsheng), & He, X. (Xiwen). (2000). A 2-aminopyridine molecularly imprinted polymer surrogate micro-column for selective solid phase extraction and determination of 4-aminopyridine. Analytica Chimica Acta, 414(1-2), 123–131. doi:10.1016/S0003-2670(00)00837-0