Aflatoxin exposure is an important public health concern in sub-Saharan Africa as well as parts of Latin America and Asia. In addition to hepatocellular carcinoma, chronic aflatoxin exposure is believed to play a role in childhood growth impairment. The most reliable biomarker of chronic aflatoxin exposure is the aflatoxin-albumin adduct, as measured by ELISA or isotope dilution mass spectrometry (IDMS). In this report, we have used high resolution LC-MS/MS with IDMS to quantitate AFB1-lysine in an extremely vulnerable population of Nigerian children suffering from severe acute malnutrition. To increase the sensitivity and reliability of the analyses, a labelled AFB1-13C6 15N2-lysine internal standard was synthesized. AFB1-lysine concentrations in this population ranged between 0.2 and 59.2 pg/mg albumin, with a median value of 2.6 pg/mg albumin. AFB1-lysine concentrations were significantly higher in stunted children (median = 4.6 pg/mg) compared to non-stunted (1.2 pg/mg), as well as in children with severe acute malnutrition (4.3 pg/mg) compared to controls (0.8 pg/mg). The median concentrations were also higher in children with kwashiorkor (6.3 pg/mg) compared to those suffering from marasmus (0.9 pg/mg). This is the first report of the use of high-resolution mass spectrometry to quantitate AFB1-lysine in humans.

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Keywords Aflatoxin, Aflatoxin-lysine adduct, High resolution LC-MS/MS, Isotope dilution mass spectrometry, Kwashiorkor, Severe acute malnutrition
Persistent URL dx.doi.org/10.1016/j.fct.2017.11.030
Journal Food and Chemical Toxicology
Citation
McMillan, A. (Amy), Renaud, J.B. (Justin B.), Burgess, K.M.N. (Kevin M.N.), Orimadegun, A.E. (Adebola E.), Akinyinka, O.O. (Olusegun O.), Allen, S.J. (Stephen J.), … Sumarah, M.W. (Mark W.). (2018). Aflatoxin exposure in Nigerian children with severe acute malnutrition. Food and Chemical Toxicology, 111, 356–362. doi:10.1016/j.fct.2017.11.030