NanoESIMS using multiple low-energy collisions was applied for observing non-covalent interactions within the nitric oxides synthase oxygenase domain. NanoESI-MS was performed using a Sciex Centaur #5 quadrupole-time of flight prototype instrument with a modified vacuum system to handle larger collision gas volumes. The results suggested that although the inducible nitric oxide synthase oxygenase domain aggregates near its calculated pH of 6.3, it is soluble between pH 2.5 and 4 and pH 6.75. It was also suggested that there is a gas phase equilibria between the positively charged dimeric and monomeric conformations of iNOS oxygenase domain.

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Conference Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Citation
Smith, J. C, Rafferty, S.P. (Steven P.), & Siu, K.W.M. (K.W. Michael). (2002). Non-covalent interactions within the nitric oxide synthase oxygenase domain observed with nanoESIMS using multiple low-energy collisions. In Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics (pp. 451–452).