Cross-linked polymers were prepared from several functional monomers and trimethylolpropane trimethacrylate (TRIM). The affinity of these functional polymers for ochratoxin A (OTA) in organic solution was evaluated by flow injection-solid phase extraction (FI-SPE) on a micro-column. Multiple loading injections of OTA through a micro-column demonstrated that N-phenylacrylamide (PAM) yielded a cross-linked polymer with the highest affinity for OTA in a variety of media. The binding capacity and binding constant were calculated for poly(TRIM-PAM). This approach has proven to be more efficient than those empirical or modeling studies reported previously. It is also time-efficient compared to obtaining Klotz and Scatchard plots from batch binding experiments. Rapid OTA screening methods were developed, using both acetonitrile and water as mobile phases in FI-SPE. A mechanism is proposed for the OTA binding, pulsed elution, and re-conditioning of poly(TRIM-PAM). The functional polymer was last evaluated for stepped elution of OTA after FI-SPE.

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Reactive and Functional Polymers
Department of Chemistry

Zhou, S.N. (Simon N.), & Lai, E.P.C. (2004). N-phenylacrylamide functional polymer with high affinity for ochratoxin A. Reactive and Functional Polymers, 58(1), 35–42. doi:10.1016/j.reactfunctpolym.2003.11.005