A finite-difference time-domain model of optical phase contrast microscope imaging
The Finite-Difference Time-Domain (FDTD) modeling technique is applied to build a simple simulation equivalent of an optical phase contrast microscope. The model is validated by demonstrating the effect of optical immersion on the optical phase contrast microscope image of a simple biological cell containing a cytoplasm, a nucleus and a membrane. To the best of our knowledge, this is the first study using the FDTD approach to construct optical phase contrast microscope images. The results demonstrate the potential of the FDTD modelling approach and extend its area of applicability a new biomedical research area.
|Keywords||Biological cell, Finite-difference time-domain method, Optical clearing effect, Optical phase contrast microscope|
|Series||NATO Science for Peace and Security Series B: Physics and Biophysics|
Tanev, S, Pond, J. (James), Paddon, P. (Paul), & Tuchin, V.V. (Valery V.). (2008). A finite-difference time-domain model of optical phase contrast microscope imaging. NATO Science for Peace and Security Series B: Physics and Biophysics. doi:10.1007/978-1-4020-6952-9-11