The Finite-Difference Time-Domain (FDTD) modeling technique is applied to build a simple simulation equivalent of an optical phase contrast microscope. The model is validated by demonstrating the effect of optical immersion on the optical phase contrast microscope image of a simple biological cell containing a cytoplasm, a nucleus and a membrane. To the best of our knowledge, this is the first study using the FDTD approach to construct optical phase contrast microscope images. The results demonstrate the potential of the FDTD modelling approach and extend its area of applicability a new biomedical research area.

Additional Metadata
Keywords Biological cell, Finite-difference time-domain method, Optical clearing effect, Optical phase contrast microscope
Persistent URL dx.doi.org/10.1007/978-1-4020-6952-9-11
Series NATO Science for Peace and Security Series B: Physics and Biophysics
Citation
Tanev, S, Pond, J. (James), Paddon, P. (Paul), & Tuchin, V.V. (Valery V.). (2008). A finite-difference time-domain model of optical phase contrast microscope imaging. NATO Science for Peace and Security Series B: Physics and Biophysics. doi:10.1007/978-1-4020-6952-9-11