The Finite-Difference Time-Domain (FDTD) approach is applied to model optical phase contrast microscope (OPCM) imaging of Gold nanoparticles (NPs) in singe biological cells. We first demonstrate the effect of optical clearing on the optical phase contrast microscope image of a realistic size biological cell containing a cytoplasm, a nucleus and a membrane. The FDTD-based OPCM model is then applied to visualize the presence of a cluster of Gold NPs in the cytoplasm at optical conditions. To the best of our knowledge, this is the first study using the FDTD approach in combination with Fourier optics techniques to construct OPCM images of realistic size cells containing NPs. The results demonstrate the potential of the FDTD modelling approach and extend its area of applicability into a new biomedical research area.

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Biophotonics: Photonic Solutions for Better Health Care
Sprott School of Business

Tanev, S, Pond, J. (James), Paddon, P. (Paul), & Tuchin, V.V. (Valery V.). (2008). FDTD simulation of optical phase contrast microscope imaging. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. doi:10.1117/12.781514