The Finite-Difference Time-Domain (FDTD) modeling technique is applied to build a simple simulation equivalent of an optical phase contrast microscope. The model is validated by demonstrating the effect of optical immersion on the optical phase contrast microscope image of a simple biological cell containing a cytoplasm, a nucleus and a membrane. To the best of our knowledge, this is the first study using the FDTD approach to construct optical phase contrast microscope images. The results demonstrate the potential of the FDTD modelling approach and extend its area of applicability a new biomedical research area.

Biological cell, Finite-difference time-domain method, Optical clearing effect, Optical phase contrast microscope
Saratov Fall Meeting 2007: Optical Technologies in Biophysics and Medicine IX
Sprott School of Business

Tanev, S, Pond, J. (James), Paddon, P. (Paul), & Tuchin, V.V. (Valery V.). (2008). Optical phase contrast microscope imaging: A FDTD modeling approach. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. doi:10.1117/12.803973