The formulation of the finite-difference time-domain (FDTD) approach is presented in the framework of its potential applications to in-vivo flow cytometry based on light scattering. The consideration is focused on comparison of light scattering by a single biological cell alone in controlled refractive-index matching conditions and by cells labeled by gold nanoparticles. The optical schematics including phase contrast (OPCM) microscopy as a prospective modality for in-vivo flow cytometry is also analyzed. The validation of the FDTD approach for the simulation of flow cytometry may open up a new avenue in the development of advanced cytometric techniques based on scattering effects from nanoscale targets.

Cytometry, Finite-difference time-domain (FDTD) method, Gold nanoparticle, Image contrast enhancement, Light scattering, Nanoscale cell imaging, Optical phase contrast microscopy, Refractive index matching
Journal of Biophotonics
Sprott School of Business

Tanev, S, Sun, W. (Wenbo), Pond, J. (James), Tuchin, V.V. (Valery V.), & Zharov, V.P. (Vladimir P.). (2009). Flow cytometry with gold nanoparticles and their clusters as scattering contrast agents: FDTD simulation of light-cell interaction. Journal of Biophotonics, 2(8-9), 505–520. doi:10.1002/jbio.200910039