The formulation of the finite-difference time-domain (FDTD) approach is presented in the framework of its potential applications to in-vivo flow cytometry based on light scattering. The consideration is focused on comparison of light scattering by a single biological cell alone in controlled refractive-index matching conditions and by cells labeled by gold nanoparticles. The optical schematics including phase contrast (OPCM) microscopy as a prospective modality for in-vivo flow cytometry is also analyzed. The validation of the FDTD approach for the simulation of flow cytometry may open up a new avenue in the development of advanced cytometric techniques based on scattering effects from nanoscale targets.

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Keywords Cytometry, Finite-difference time-domain (FDTD) method, Gold nanoparticle, Image contrast enhancement, Light scattering, Nanoscale cell imaging, Optical phase contrast microscopy, Refractive index matching
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Journal Journal of Biophotonics
Tanev, S, Sun, W. (Wenbo), Pond, J. (James), Tuchin, V.V. (Valery V.), & Zharov, V.P. (Vladimir P.). (2009). Flow cytometry with gold nanoparticles and their clusters as scattering contrast agents: FDTD simulation of light-cell interaction. Journal of Biophotonics, 2(8-9), 505–520. doi:10.1002/jbio.200910039