1. 1. Mitochondria with high respiratory control ratios (RCR) have been isolated from the ventricle of the marine clam Mercenaria mercenaria. 2. 2. Proline is the preferred substrate of the mitochondria of the ventricle based on state 3 rates. 3. 3. Pyruvate, ornithine and succinate are oxidized at rates 3/4 that of proline. 4. 4. α-Glycerophosphate was oxidized at rates 1/2 that of proline. 5. 5. The pH optimum for proline oxidation lies between 6.5 and 7.5 based on RCR and ADP/O and between 7.0 and 7.4 based on state 3 rates. 6. 6. KCl concentrations between 250 and 450 mM gave optimal values for the oxidation of proline based on RCR and state 3 rates. 7. 7. KCl concentration had little effect on ADP/O between 100 and 850 mM.

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Persistent URL dx.doi.org/10.1016/0305-0491(83)90184-0
Journal Comparative Biochemistry and Physiology -- Part B: Biochemistry and
Citation
Ballantyne, J.S., & Storey, K. (1983). Mitochondria from the ventricle of the marine clam, Mercenaria mercenaria: Substrate preferences and effects of pH and salt concentration on proline oxidation. Comparative Biochemistry and Physiology -- Part B: Biochemistry and, 76(1), 133–138. doi:10.1016/0305-0491(83)90184-0