Camelid single-domain antibodies raised by DNA immunization are potent inhibitors of EGFR signaling
Biochemical Journal , Volume 476 - Issue 1 p. 39- 50
Up-regulation of epidermal growth factor receptor (EGFR) is a hallmark of many solid tumors, and inhibition of EGFR signaling by small molecules and antibodies has clear clinical benefit. Here, we report the isolation and functional characterization of novel camelid single-domain antibodies (sdAbs or VHHs) directed against human EGFR. The source of these VHHs was a llama immunized with cDNA encoding human EGFR ectodomain alone (no protein or cell boost), which is notable in that genetic immunization of large, outbred animals is generally poorly effective. The VHHs targeted multiple sites on the receptor’s surface with high affinity (KD range: 1–40 nM), including one epitope overlapping that of cetuximab, several epitopes conserved in the cynomolgus EGFR orthologue, and at least one epitope conserved in the mouse EGFR orthologue. Interestingly, despite their generation against human EGFR expressed from cDNA by llama cells in vivo (presumably in native conformation), the VHHs exhibited wide and epitope-dependent variation in their apparent affinities for native EGFR displayed on tumor cell lines. As fusions to human IgG1 Fc, one of the VHH-Fcs inhibited EGFR signaling induced by EGF binding with a potency similar to that of cetuximab (IC50: ∼30 nM). Thus, DNA immunization elicited high-affinity, functional sdAbs that were vastly superior to those previously isolated by our group through protein immunization.
|Organisation||Department of Biology|
Rossotti, M.A. (Martin A.), Henry, K.A. (Kevin A.), van Faassen, H. (Henk), Tanha, J. (Jamshid), Callaghan, D. (Deborah), Hussack, G. (Greg), … Roger MacKenzie, C. (C.). (2019). Camelid single-domain antibodies raised by DNA immunization are potent inhibitors of EGFR signaling. Biochemical Journal, 476(1), 39–50. doi:10.1042/BCJ20180795