Cloning and expression of hypoxia-inducible factor 1α from the hibernating ground squirrel, Spermophilus tridecemlineatus
Biochimica et Biophysica Acta - Gene Structure and Expression , Volume 1729 - Issue 1 p. 32- 40
Mammalian hibernation is associated with apnoic breathing patterns and a hypoxia-hypothermia connection has been suggested as part of the mechanism by which body temperature is reduced as animals enter torpor. Hence, we hypothesized that changes in the expression of the hypoxia inducible factor (HIF-1) may potentially be involved in regulating hibernation-responsive gene targets. The expression of the alpha subunit of HIF-1 was quantified at both gene and protein levels in four organs of the thirteen-lined ground squirrel, Spermophilus tridecemlineatus. Reverse transcription-PCR showed no change in hif-1α transcript levels in the liver, lung, skeletal muscle or brown adipose tissue of euthermic versus hibernating animals but HIF-1α protein levels were elevated by 60-70% in the two organs responsive for thermogenesis (brown adipose and skeletal muscle). Furthermore, assessment of DNA binding by HIF-1 in nuclear extracts from brown adipose revealed 6-fold higher levels in hibernator tissue than in euthermic controls suggesting increased expression of HIF-1 responsive genes during hibernation. The complete nucleotide sequence of hif-1α from ground squirrels, the first hif-1α sequence amplified from a hibernating mammal, was obtained using PCR amplification and 3′ and 5′ RACE. Amino acid sequence analysis revealed 90-95% identity with the HIF-1α protein from other mammals. Several unique amino acid sequence substitutions were identified that may affect protein conformation and could possibly function to counteract low temperature effects on HIF-1α conformation at near 0°C body temperatures during torpor.
|13-Lined ground squirrel, Brown adipose tissue, Hypoxia-inducible factor 1, Mammalian hibernation, Muscle, Thermogenesis|
|Biochimica et Biophysica Acta - Gene Structure and Expression|
|Organisation||Department of Chemistry|
Morin Jr., P. (Pier), & Storey, K. (2005). Cloning and expression of hypoxia-inducible factor 1α from the hibernating ground squirrel, Spermophilus tridecemlineatus. Biochimica et Biophysica Acta - Gene Structure and Expression, 1729(1), 32–40. doi:10.1016/j.bbaexp.2005.02.009