In the present study, the seed oil from Onopordum acanthium. L and methanolic extract of the defatted meal were evaluated for their cytotoxic effect and antioxidant potential. The phenolic composition of the defatted meal was determined with RP-HPLC-UV and tocopherols levels in the crude oil were also assessed. Overall, the methanolic extract exhibited the best amount of total phenolics and flavonoids content (78.06±0.55 mg GAE/g on DW basis and 20.38±0.21 mg RE/g on DW basis, respectively).Seven phenolic compounds were identified with cinnamic acids as the major components (67.2%) and epicatechin was the dominant flavan-3-ol. Additionally, three individual tocopherols were determined and α-tocopherol was the main isomer (914.8±0.02mg/kg). Data of antioxidant performance showed superior antioxidant capacity in methanol extract. Moreover methanolic extract from defatted seeds was more active towards HepG2 cancer cells than the crude oil(IC50 of 44.05±3.54µg/ml) and showed less cytotoxicity towards AML12 normal hepatocytes in comparison to doxorubicin indicating at least some tumor-specific action.

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Journal Revue Roumaine de Chimie
Oueslati, M.A. (Moufida A.), Gunenc, A. (Aynur), Rigane, G. (Ghayth), Ghazghazi, H. (Hanen), Valencia, C. (Caroline), Ben Salem, R. (Ridha), … Hosseinian, F. (2019). Chemical composition, antioxidant and cytotoxic activities of Onopordum acanthium L. Crude oil and defatted meal. Revue Roumaine de Chimie, 64(6), 503–510. doi:10.33224/rrch/2019.64.6.06