This study was undertaken to clarify the physiological role of catalase in the maintenance of pro/antioxidant balance in goldfish tissues by inhibiting the enzyme in vivo with 3-amino 1,2,4-triazole. Intraperitoneal injection of aminotriazole (0.5 mg/g wet mass) caused a decrease in liver catalase activity by 83% after 24 h that was sustained after 168 h post-injection. In kidney catalase activity was reduced by ∼50% and 70% at the two time points, respectively. Levels of protein carbonyls were unchanged in liver but rose by 2-fold in kidney after 168 h. Levels of thiobarbituric acid-reactive substances were elevated in both tissues after 24 h but were reversed by 168 h. Glutathione peroxidase and glutathione-S-transferase activities increased in kidney after aminotriazole treatment whereas activities of glutathione peroxidase and glutathione reductase in liver decreased after 24 h but rebounded by 168 h. Liver glucose-6-phosphate dehydrogenase activity was reduced at both time points. Activities of these three enzymes in liver correlated inversely with the levels of lipid damage products (R2 = 0.65-0.81) suggesting that they may have been oxidatively inactivated. Glutathione-S-transferase activity also correlated inversely with catalase (R2 = 0.86). Hence, the response to catalase depletion involves compensatory changes in the activities of enzymes of glutathione metabolism.

Additional Metadata
Keywords 3-Amino 1,2,4-triazole, Carassius auratus, Carbonyl proteins, Glutathione peroxidase, Glutathione-S-transferase, Kidney, Liver, Thiobarbituric reactive substances
Persistent URL dx.doi.org/10.1016/j.cbpb.2005.08.003
Journal Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Citation
Bagnyukova, T.V. (Tetyana V.), Storey, K, & Lushchak, V.I. (Volodymyr I.). (2005). Adaptive response of antioxidant enzymes to catalase inhibition by aminotriazole in goldfish liver and kidney. Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, 142(3), 335–341. doi:10.1016/j.cbpb.2005.08.003