Currently, there are no time-saving and cost-effective high-throughput screening methods for the evaluation of bacterial drug-resistance. In this study, a droplet microarray (DMA) system is established as a miniaturized platform for high-throughput screening of antibacterial compounds using the emerging, opportunistic human pathogen Pseudomonas aeruginosa (P. aeruginosa) as a target. Based on the differences in wettability of DMA slides, a rapid method for generating microarrays of nanoliter-sized droplets containing bacteria is developed. The bacterial growth in droplets is evaluated using fluorescence. The new method enables immediate screening with libraries of antibiotics. A novel simple colorimetric readout method compatible with the nanoliter size of the droplets is established. Furthermore, the drug-resistance of P. aeruginosa 49, a multi-resistant strain from an environmental isolate, is investigated. This study demonstrates the potential of the DMA platform for the rapid formation of microarrays of bacteria for high-throughput drug screening.

Additional Metadata
Keywords antibiotic resistance screening, droplet-microarray, facultative pathogenic Pseudomonas aeruginosa
Persistent URL dx.doi.org/10.1002/adbi.202000073
Journal Advanced Biosystems
Citation
Lei, W. (Wenxi), Demir, K. (Konstantin), Overhage, J, Grunze, M. (Michael), Schwartz, T. (Thomas), & Levkin, P.A. (Pavel A.). (2020). Droplet-Microarray: Miniaturized Platform for High-Throughput Screening of Antimicrobial Compounds. Advanced Biosystems. doi:10.1002/adbi.202000073