Characterization of quantum dots using capillary zone electrophoresis
Commercially available quantum dots (QDs) were characterized using CE. The CE instrument were laboratory-built, each being capable of both electrokinetic and hydrodynamic injection. Modes of detection include UV absorption and LIF. The CE-LIF system was further modified to handle microliter sample volumes during injection. Sodium phosphate (5-25 mM, pH 7.5-11) was found to be a good buffer electrolyte. Sodium mercaptoproprionate CdTe/CdS (ADS620) QDs and carboxylic add CdSe/ZnS (T2-Evitag) QDs yielded high separation efficiencies of N = 1.5 × 106 plates at tM = 10 min and N = 1.0 × 105 plates at tM = 3.8 min, respectively. Apparently the EDC/sulfo-NHS bioconjugation chemistry worked well with the neutral T2-Evitag QDs, but not so well with the negatively charged ADS620 QDs. This preliminary knowledge will serve as a basis for new CE immunoassay studies of QD-biomolecule conjugates and their immunocomplexes with target analytes.
|Keywords||CE, LIF, Micro-sample injection, Migration time characterization, Quantum dots|
Pereira, M. (Mark), Lai, E. P, & Hollebone, B. (Bryan). (2007). Characterization of quantum dots using capillary zone electrophoresis. Electrophoresis, 28(16), 2874–2881. doi:10.1002/elps.200600737