A novel micro-solid phase extraction (μSPE) device was prepared by electrochemically adhering molecularly imprinted polypyrrole encapsulated carbon nanotubes (MIPPy/CNTs) onto a stainless steel frit. It was found that the use of CNTs increased the total binding capacity without compromising the porosity of the frit. Three templates, 17 α-ethynyl estradiol (EE2), 17β-estradiol (E2), and ochratoxin A (OTA) were imprinted individually to make their respective MIPPy/CNTs-modified frits. The binding properties of these μSPE devices were characterized by high performance liquid chromatography (HPLC) with fluorescence detection (FLD). In comparison with non-imprinted polypyrrole encapsulated carbon nanotubes (PPy/CNTs), the molecular imprinting technique enhanced the selective binding towards the imprint molecules. MIPPy/CNTs-/μSPE-HPLC-FLD results demonstrated the selectivity of MIPPy had been tailored by imprinting with different templates. This is the first report on how versatile pyrrole can be as a functional monomer for the molecular imprinting of target compounds with a broad range of high to low pKa values. Copyright

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Journal of Nanoscience and Nanotechnology
Department of Chemistry

Yu, J.C.C. (Jörn C. C.), Hrdina, A. (Amy), Mancini, C. (Christina), & Lai, E.P.C. (2007). Molecularly imprinted polypyrrole encapsulated carbon nanotubes in stainless steel frit for micro solid phase extraction of estrogenic compounds. Journal of Nanoscience and Nanotechnology, 7(9), 3095–3103. doi:10.1166/jnn.2007.666