EF-P (eubacterial elongation factor P) is a highly conserved protein essential for protein synthesis. We report that EF-P protects 16S rRNA near the G526 streptomycin and the S12 and mRNA binding sites (30S T-site). EF-P also protects domain V of the 23S rRNA proximal to the A-site (50S T-site) and more strongly the A-site of 70S ribosomes. We suggest that EF-P: (a) may play a role in translational fidelity and (b) prevents entry of fMet-tRNA into the A-site enabling it to bind to the 50S P-site. We also report that EF-P promotes a ribosome-dependent accommodation of fMet-tRNA into the 70S P-site.

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FEBS Journal
Department of Biology

Aoki, H. (Hiroyuki), Xu, J. (John), Emili, A. (Andrew), Chosay, J.G. (John G.), Golshani, A, & Ganoza, M.C. (M. Clelia). (2008). Interactions of elongation factor EF-P with the Escherichia coli ribosome. FEBS Journal, 275(4), 671–681. doi:10.1111/j.1742-4658.2007.06228.x