Programmed cell death (PCD) was triggered in wheat leaves by a heat treatment (42°C). This treatment caused DNA fragmentation as shown in DNA laddering analysis and in terminal deoxynucleotidyl transferase-mediated deoxyuridine-5′-triphosphate (dUTP) nick end labeling (TUNEL) analysis. Methanol and acetone treatment of leaves significantly blocked PCD. Western analysis indicated that a 65 kDa poly(ADP-ribose) polymerase-like protein was degraded during the treatment. However, high temperature (80°C) treatment caused necrosis but not PCD.

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Keywords caspase-like protease, DNA ladder, necrosis, PARP, TUNEL
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Journal Biologia Plantarum
Fan, T., & Xing, T. (2004). Heat shock induces programmed cell death in wheat leaves. Biologia Plantarum, 48(3), 389–394. doi:10.1023/