Successful targeted imaging of BxPC3 human pancreatic cancer cells is feasible with near-IR CdTeSe/CdS quantum dots (QDs) functionalized with single-domain antibody (sdAb) 2A3. For specific targeting, sdAbs are superior to conventional antibodies, especially in terms of stability, aggregation, and production cost. The bright CdTeSe/CdS QDs were synthesized to emit in the diagnostic window of 650-900 nm with a narrow emission band. 2A3 was derived from llama and is small in size of ∼13 kDa, but with fully-functional recognition to the target carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6), a possible biomarker as a therapeutic target of pancreatic cancer. For compelling imaging, optical may be the most sensible among the various imaging modalities, regarding the sensitivity and cost. This first report on sdAb-conjugated near-IR QDs with high signal to background sensitivity for targeted cellular imaging brings insights into the development of optical molecular imaging for early stage cancer diagnosis. Copyright

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Keywords Colloidal nanocrystals, In vitro targeted cellular imaging, Pancreatic and breast cancer, Photoluminescent near-IR quantum dots (QDS), Single-domain antibody
Persistent URL dx.doi.org/10.1166/jnn.2011.4167
Journal Journal of Nanoscience and Nanotechnology
Citation
Zaman, M.B. (Md. Badruz), Baral, T.N. (Toya Nath), Jakubek, Z.J. (Zygmunt J.), Zhang, J. (Jianbing), Wu, X. (Xiaohua), Lai, E. P, … Yu, K. (Kui). (2011). Single-domain antibody bioconjugated near-IR quantum dots for targeted cellular imaging of pancreatic cancer. In Journal of Nanoscience and Nanotechnology (Vol. 11, pp. 3757–3763). doi:10.1166/jnn.2011.4167