Successful targeted imaging of BxPC3 human pancreatic cancer cells is feasible with near-IR CdTeSe/CdS quantum dots (QDs) functionalized with single-domain antibody (sdAb) 2A3. For specific targeting, sdAbs are superior to conventional antibodies, especially in terms of stability, aggregation, and production cost. The bright CdTeSe/CdS QDs were synthesized to emit in the diagnostic window of 650-900 nm with a narrow emission band. 2A3 was derived from llama and is small in size of ∼13 kDa, but with fully-functional recognition to the target carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6), a possible biomarker as a therapeutic target of pancreatic cancer. For compelling imaging, optical may be the most sensible among the various imaging modalities, regarding the sensitivity and cost. This first report on sdAb-conjugated near-IR QDs with high signal to background sensitivity for targeted cellular imaging brings insights into the development of optical molecular imaging for early stage cancer diagnosis. Copyright

Additional Metadata
Keywords Colloidal nanocrystals, In vitro targeted cellular imaging, Pancreatic and breast cancer, Photoluminescent near-IR quantum dots (QDS), Single-domain antibody
Persistent URL
Journal Journal of Nanoscience and Nanotechnology
Zaman, M.B. (Md. Badruz), Baral, T.N. (Toya Nath), Jakubek, Z.J. (Zygmunt J.), Zhang, J. (Jianbing), Wu, X. (Xiaohua), Lai, E. P, … Yu, K. (Kui). (2011). Single-domain antibody bioconjugated near-IR quantum dots for targeted cellular imaging of pancreatic cancer. In Journal of Nanoscience and Nanotechnology (Vol. 11, pp. 3757–3763). doi:10.1166/jnn.2011.4167