The objective of this study was to investigate the effects of the herbicide 3-amino-1,2,4-triazole (AMT) on the activities of catalase and lactate dehydrogenase (LDH) in blood (plasma and erythrocytes) and eight solid tissues of goldfish, Carassius auratus. Injection of goldfish with AMT (0.5. mg/gww AMT in 0.9% NaCl) resulted in a significant decrease in catalase activity 24. h post-injection in most tissues investigated. In white and red muscle, kidney, heart, liver, brain and erythrocytes the activity of catalase decreased by 61%, 69%, 64%, 48%, 40%, 27% and 26%, respectively, in comparison to the values seen in animals injected with physiological saline (0.9% NaCl). However, the activity of LDH decreased only in red muscle (by 19%) after AMT injection, whereas in plasma it increased by 137%. Protein carbonyl levels, a measure of oxidative damage to proteins, did not change in plasma in goldfish injected with AMT and total hemoglobin levels in AMT-injected fish, although lower compared with uninjected controls, did not differ from values in saline-injected controls. It is proposed that catalase activity in erythrocytes and white muscle might be usefully developed as a potential marker for fish intoxication by aminotriazole and other related herbicides.

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Keywords 3-Amino-1,2,4-triazole, Carassius auratus, Catalase, Erythrocytes, Hemoglobin, Lactate dehydrogenase, Plasma
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Journal Pesticide Biochemistry and Physiology
Vasylkiv, O.Y. (Olena Yu.), Kubrak, O.I. (Olga I.), Storey, K, & Lushchak, V.I. (Volodymyr I.). (2011). Catalase activity as a potential vital biomarker of fish intoxication by the herbicide aminotriazole. Pesticide Biochemistry and Physiology, 101(1), 1–5. doi:10.1016/j.pestbp.2011.05.005