In this study, medium oat bran samples were treated with four cell wall carbohydrases degrading enzymes (viscozyme® L, α-amylase, amyloglucosidase, celluclast®). Their effects on protein extraction efficiency and, on antioxidant properties of obtained protein isolates and resulting alcalase hydrolysates were investigated. The results showed that sample pre-treated with amyloglucosidase (8 units/g) had the higher protein content (82%) while celluclast (5-60 endo-glucanase units (EGU)/g) pre-treated samples had protein content similar to that of control (54%). In the oxygen radical absorbance assay (ORAC) all samples of protein isolates had significantly higher peroxyl radical scavenging activity than the control. The sample pre-treated with alpha-amylase (2300 units/g) displayed the highest activity with an ORAC value of 165 ± 16 μM Trolox Equivalents (TE)/g. Proteins were hydrolyzed with alcalase and radical scavenging activities of hydrolysates assessed by ORAC and HO• radical assays. Sample pre-treated with viscozyme 1.5 Fungal Beta-glucanase Units (FBU)/g was the more active in both assays: ORAC value 521 ± 53 μM TE/g and HO• radical inhibition 26.3 ± 1.5%. A tandem mass spectrometric analysis of multiple charged ions within the hydrolysates allowed the identification of four new peptides.

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Keywords Antioxidant, Carbohydrase, Mass spectrometry, Oat bran, Peptide
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Journal Food Research International
Jodayree, S. (Sara), Smith, J. C, & Tsopmo, A. (2012). Use of carbohydrase to enhance protein extraction efficiency and antioxidative properties of oat bran protein hydrolysates. Food Research International, 46(1), 69–75. doi:10.1016/j.foodres.2011.12.004