The ability of enzymes to catalyze specific reactions, while excluding others, is central to cellular metabolism. Control of reaction specificity is of particular importance for enzymes that employ catalytically versatile cofactors, of which pyridoxal 5′-phosphate is a prime example. Cystathionine γ-synthase and cystathionine β-synthase are the first enzymes in the transsulfuration and reverse transsulfuration pathways, respectively. Each of them occupies branch-point positions in amino acid metabolism and as such are subject to transcriptional and post-translational regulation. Both enzymes catalyze the pyridoxal 5′-phosphate-dependent formation of l-cystathionine; however, their substrate and reaction specificities are distinct. The mechanisms whereby these enzymes control the chemistry of the cofactor are the subject of this review.

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Keywords Cystathionine, Cysteine, Homocysteine, Methionine, Pyridoxal 5′-phosphate, Reaction specificity, Sulfur amino acid
Persistent URL dx.doi.org/10.1016/j.abb.2004.08.024
Journal Archives of Biochemistry and Biophysics
Citation
Aitken, S, & Kirsch, J.F. (Jack F.). (2005). The enzymology of cystathionine biosynthesis: Strategies for the control of substrate and reaction specificity. Archives of Biochemistry and Biophysics, 433(1), 166–175. doi:10.1016/j.abb.2004.08.024